Lack of Association between fbe Gene with Adherence and Biofilm Forrr：ation in Staphylococcus epidermidis Clinical Isolates

Biofilm formation plays a major role in the pathogenesis of nosocomial infections caused by Staphylococcus epidermidis (S. epidermidis). It has been suggested that protein encoded by the foe (fibrinogen binding protein) gene of S. epidermidis enhances bacterial adherence to medical devices and biofilm formation by binding to host fibrinogen (Fg). In this study, a 1.7 kbfoe gene fragment was amplified in 111 of 115 strains of S. epidermidis chnical isolates using PCR. Contrary to expectations, only 14 strains showed marginally increased adherence to Fg-coated polystyrene wells compared with BSA coated wells. Quantitative real-time PCR revealed no statistically significant difference in Fbe expression between Fg binding strains and Fg non-binding strains. Fttahermore, in the presence of soluble Fg, S. epidermdis biofilm formation decreased in a dose-dependent manner. In contrast, the Staphylococcus aureus ( S. aureus ) strain Cowan I and other 5 S. aureus clinical isolates showed a substantial increase in both adherence and biofilm formation in the presence of Fg. The resuits suggest that in S. epidermidis the foe gene may not be associated with bacterial adherence and biofilm formation.     

对左旋多巴长期治疗帕金森病的评价

很多研究证明长期应用左旋多巴(L-dopa)治疗帕金森病(PD),可明显改善PD患者的症状,而且应该在PD的早期应用适量的左旋多巴进行治疗,以改善患者的早期症状。长期应用左旋多巴治疗PD与长期应用多巴胺受体激动剂对疾病导致的病死率相同。长期应用多巴胺受体激动剂治疗PD的疗效与长期应用左旋多巴相同,前者并未显示出优越性。     

大花红天素对脑微血管内皮细胞凋亡的双相调节作用及机制(英文)

目的：研究中药红景天的有效成分-大花红天素对小鼠脑微血管内皮细胞凋亡的作用及机制。 方法： 观察不同剂量的大花红天素(25 mg/L, 100 mg/L)对体外培养的小鼠脑微血管内皮细胞(bEnd.3株)凋亡的影响。细胞凋亡分别用流式细胞术、免疫细胞化学ABC法(Fas/Bcl-2)及Western blotting(caspase-3)测定。 结果： 与对照组比，在bEnd.3细胞中含药浓度25 mg/L组凋亡明显抑制(P<0.05)，而100 mg/L组凋亡明显加剧(P<0.05)。凋亡抑制组Fas免疫细胞化学染色比对照组弱，阳性细胞明显减少(P<0.05)；而Bcl-2染色比对照组强，阳性细胞明显增加(P<0.05)，caspase-3表达明显抑制(P<0.05)。凋亡加剧组中(100 mg/L)变化相反。 结论： 大花红天素对bEnd.3细胞凋亡有双相调节作用，其机制与Fas/Bcl-2蛋白表达及caspase-3激活的变化有关。     

早期慢性肾病患者动态血压变化与左心室肥厚的关系

目的：分析早期慢性肾脏病（CKD1期）患者24h动态血压变化与左心室肥厚（LVH）的关系。方法：以25例正常人作为对照组（N组）,71例肾功能稳定的CKD1期患者作为疾病组（D组）。收集肾功能、血脂、24h动态血压监测（ABPM）等临床资料;采用超声心动图检测早期CKD患者LVH有关指标,分析ABPM指标与LVH的关系。结果：（1）与N组相比,D组夜间收缩压,昼、夜及24h平均舒张压均升高（P均〈0.05）;夜间收缩压下降率（nDRS）及舒张压下降率（nDRD）均明显下降（P均〈0.05）;舒张末期左室内径（LVDd）及左心室质量指数（LVMI）均升高（P均〈0.05）。（2）D组高血压及非杓型血压发生率分别达47.9%、62.0%。（3）与杓型血压组（Dip组）相比,非杓型血压组（non-Dip组）LVMI值及LVH发生率均显著增高（P均〈0.05）。（4）与非高血压组（non-LVH组）相比,高血压组（LVH组）nDRS和nDRD均明显下降,血红蛋白（Hb）显著降低（P均〈0.05）。（5）相关性分析显示LVMI值与nDRS、nDRD和Hb均呈负相关（P均〈0.01）,昼间平均收缩压（dSBP）、夜间平均收缩压（nSBP）、夜间平均舒张压（nDBP）和24h平均舒张压（mSBP）均呈正相关（P均〈0.05）。多因素逐步回归分析显示：nDRS、Hb、nDRD和血肌酐（Scr）进入回归方程：y=123.429-2.290x1-0.47x2-0.768x3＋0.178x4（y=LVMI;123.429=常数,t=8.41,P=0.000;x1=nDRS,t=-5.43,P=0.000;x2=Hb,t=-4.77,P=0.000;x3=NDRD,t=-3.47,P=0.001;x4=Scr,t=2.08,P=0.041）。结论：早期CKD患者即已出现血压升高及血压节律改变;LVH发生与早期CKD患者夜间高血压及非杓型血压关系更为密切;贫血和肾功能减退本身也与早期CKD患者LVH发生有关。     

超声心动图在心脏移植中的应用进展

心脏移植是治疗终末期严重心脏病最有效的方法,但排异反应(主要为心脏移植体的血管病)仍是影响患者长期生存的主要因素.心内膜心肌活检、冠状动脉造影与血管内超声等有创方法不但费时,而且不适合长期监测.超声心动图技术具有无创、方便等优点,在临床应用中具独特的优势.组织多普勒、三维超声心动图、斑点追踪技术、负荷及造影超声心动图等新技术及相互融合比较被证实在心脏排异反应的筛查中具有重要价值.     

Effects of Transdermal Estrogen Therapy on Expressions of Estrogen Receptors and T-lymphocyte Apoptosis in Surgically Menopausal Women

Studies have demonstrated estrogen replacement therapy can improve the life quality of surgically menopausal women. However, the mechanisms in this process remain poorly defined. Here we show the effect of transdermal estrogen therapy on expressions of estrogen receptors and T-lymphocyte apoptosis in surgically postmenopausal women. Fifteen surgically menopausal women, 15 naturally menopausal women and 15 young women were chosen in our studies. Peripheral vein blood was collected and serum E2 and FSH levels were assessed using ACCESS. T-lymphocyte apoptosis and the expressions of Fas, FasL and ER subtypes α and β were determined. The serum E2 levels of surgically menopausal woman were significantly higher, and the ＂Improved Kupperman Index＂ and the scores of ＂Menopause Specific Quality of Life Questionnaire＂ in surgically menopausal women were significantly low after ERT. The rates of T-lymphocyte apoptosis and FasL expression in surgically menopausal women were decreased after ERT, but the difference was not significant. The expressions of ERa and ERβ in two menopausal groups were significantly lower than those of the young group. They were both significantly up-regulated after 3 months of ERT. Transdermal ERT could significantly upregulate the serum E2 level, could improve menopausal symptoms and life quality of surgically menopausal women and upregulate ERa and ERβ expressions on T lymphocytes, especially ERp. Thus, the low dose of transdermal ERT may have a protective effect on menopausal women＇s immune function and aging.     

Efficient delivery of human clotting factor IX after injection of lentiviral vectors in utero

AIM: To explore gene transfer feasibility for human clotting factor IX (hFIX) mediated by recombinant lentivirus in utero. METHODS: ICR mice fetus at 17-19 d gestation were received lentiviral vectors carrying hFIX cDNA under the control of liver specific promoter by intrahepatic injection. The expression and distribution of hFIX cDNA and possible immune responses against the hFIX were assessed by ELISA, PCR, RT-PCR, and immunohistochemistry, respectively. RESULTS: The serum hFIX protein were detected at different time points in all newborn mice, the highest level of hFIX was 50 μg/L and lasted for more than 30 d. Anti-hFIX antibody was not detected, hFIX cDNA was detected in liver, spleen, and heart. The expression of hFIX cDNA was only detected in liver. Besides, no germ line transmission was found at DNA and RNA levels, and no side effect associated with gene transfer was detected. CONCLUSION: The efficient delivery of hFIX can be achieved by prenatal gene transfer. It thus shows the feasibility of gene therapy for hemophilia in utero.     

PKB在类胰蛋白酶诱导基因表达中的作用

目的：探讨蛋白激酶B(PKB)在类胰蛋白酶（tryptase）诱导基因表达中的作用。 方法： 采用RT-PCR和Western blotting 方法，检测tryptase对ECV304细胞PKB（Akt）的表达及其活性和对转录因子AP-1、NF-κB p65亚单位、JNK、p38MAPK、趋化因子IL-8表达的影响。 结果： 在ECV304中1 μg/L tryptase可使PKB蛋白质磷酸化水平增加并促进PKB、转录因子NF-κB P65亚单位、AP-1和趋化因子IL-8的表达，但对JNK、p38MAPK表达影响不大。PI3K特异性抑制剂LY294002可抑制PKB的表达增加，同时可抑制NF-κB P65亚单位和IL-8的表达增加；反义PKB质粒瞬时转染ECV304，可抑制PKB、AP1、NF-κB P65亚单位和IL-8的表达增加；PAR2的抗体可抑制PKB的磷酸化，但不能阻断PKB表达。 结论： 在ECV304细胞tryptase经其膜受体PAR2通过PI3K促进PKB的磷酸化而激活之，通过其下游途径促进趋化因子IL-8、转录因子AP-1、NF-κB P65亚单位和PKB本身的表达增加。